| 產品名稱 : | PH0713 | Tris堿 三羥甲基氨基甲烷 TRIS/Tris-base/THAM CAS:77-86-1 |
| 產品品牌 : | 飛凈 PHYGENE |
貨號 PH0713
名稱 Tris-Base(三羥甲基氨基甲烷)
規格 100g | 500g | 1000g
保存 常溫保存,長期有效
產品簡介
Tris base,即Tris堿,中文名也稱為三(羥甲基)氨基甲烷,即生物化學和分子生物學中常常被配制成多種緩沖液,例如:Tris-EDTA(TE緩沖液),多用于核酸的純化和儲存,保護其不被核酸酶降解;Tris acetate-EDTA(TAE緩沖液)和Tris borate-EDTA(TBE緩沖液)普遍用作凝膠配制緩沖液以及電泳緩沖液。
Tris緩沖液不僅被廣泛用作核酸和蛋白質的溶劑,還有許多重要用途。Tris被用于不同pH條件下的蛋白質晶體生長。Tris緩沖液的低離子強度特點可用于線蟲(C. elegans)核纖層蛋白(lamin)的中間纖維的形成。Tris也是蛋白質電泳緩沖液的主要成分之一。此外,Tris還是制備表面活性劑、硫化促進劑和一些藥物的中間物。Tris也被用作滴定標準物。
緩沖特性
Tris為弱堿,在室溫(25℃)下,它的pKa為8.1;根據緩沖理論,Tris緩沖液的有效緩沖范圍在pH7.0到9.2之間。
Tris堿的水溶液pH在10.5左右,一般加入鹽酸以調節pH值至所需值,即可獲得該pH值的緩沖液。但同時應注意溫度對于Tris的pKa的影響。
產品性質
中文別名(Chinese synonym) 2-氨基-2-(羥甲基)-1,3-丙二醇;Tris 堿;氨基丁三醇;三(羥甲基)氨基甲烷;
英文別名(English synonym) THAM; Trizma base; Trometamol; Tris(hydroxymethyl)aminomethane;
2-Amino-2-(hydroxymethyl)-1,3-propanediol;
CAS號(CAS NO.) 77-86-1
分子式(Formula) NH2C(CH2OH)3
分子量(Molecular weight) 121.14
外觀(Appearance) 白色結晶粉末至針狀結晶
熔點(Melting point) 168-172 °C
溶解性(Solubility) 溶于水
純度 99.9%
注意事項
為了您的安全和健康,請穿實驗服并戴一次性手套操作。
Tris, or tris(hydroxymethyl)aminomethane, or known during medical use as tromethamine or THAM, is an organic compound with the formula (HOCH2)3CNH2. It is extensively used in biochemistry and molecular biology as a component of buffer solutions such as in TAE and TBE buffers, especially for solutions of nucleic acids.
Buffering features
The conjugate acid of Tris has a pKa of 8.07 at 25 °C, which implies that the buffer has an effective pH range between 7.1 and 9.1 (pKa ± 1) at room temperature.
Buffer details
The pKa declines approximately 0.03 units per degree Celsius rise in temperature.
Silver-containing single-junction pH electrodes (e.g., silver chloride electrodes) are incompatible with tris since an Ag-tris precipitate forms which clogs the junction. Double-junction electrodes are resistant to this problem, and non-silver containing electrodes are immune.
Other names
TRIS, Tris, Tris base, Tris buffer, Trizma, Trisamine, THAM, Tromethamine, Trometamol, Tromethane, Trisaminol
溫馨提示:1.本產品僅供科研使用。請勿用于醫藥、臨床診斷或治療,食品及化妝品等用途。請勿存放于普通住宅區。2.為了您的安全和健康,請穿好實驗服并佩戴一次性手套和口罩操作。3.實驗結果可由多種因素影響,相關處理只限于產品本身,不涉及其他賠償。
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