貨號 PH0620

名稱 TE 緩沖液 Tris-EDTA buffer（pH 8.0）

規格 100ML | 500ML

存儲 室溫保存

產品簡介 

TE即Tris-EDTA buffer，也稱為T10E1 buffer，是分子生物學的常用緩沖溶液，尤其是涉及到DNA，RNA的過程中保護其不被降解。TE緩沖液由Tris（最常用的pH緩沖液）和EDTA（二價金屬離子螯合劑）兩種試劑組成，前者常用的pH緩沖范圍為7.5-8.5，這種環境下核酸酶的活性較低；后者通過螯合核酸酶所必需的金屬離子，從而進一步使核酸酶失活。

本品是即用型的1×TE緩沖液，pH 8.0，經高壓滅菌處理，不含DNase酶，適用于DNA的純化和保存。也可用作清洗緩沖液（washing buffer）。

產品性質 1×TE工作液含有10 mM Tris-HCl 和1 mM EDTA·Na2，pH 8.0

注意事項 

為了您的安全和健康，請穿實驗服并戴一次性手套操作。

TE buffer is a commonly used buffer solution in molecular biology, especially in procedures involving DNA, cDNA or RNA. "TE" is derived from its components: Tris, a common pH buffer, and EDTA, a molecule that chelates cations like Mg2+. The purpose of TE buffer is to solubilize DNA or RNA, while protecting it from degradation.

Tris buffer solutions are widely used in cell and molecular biology for processes such as protein and nucleic acid extraction and purification. Tris-EDTA (TE) buffer solution, pH 8.0 may also be used as a washing buffer.

Short-term storage (weeks) 4 degree centigrade in Tris-EDTA
Medium-term storage (months) -80 degree centigrade in Tris-EDTA
Long-term storage (years) -80 degree centigrade as a precipitate in ethanol
Long-terms storage (decades) -164 degree centigrade or dried.