貨號(hào) PH0613

名稱 Tris-硼酸電泳緩沖液（5×TBE）

規(guī)格 500mL

存儲(chǔ) 室溫儲(chǔ)存，有效期至少12 個(gè)月。

產(chǎn)品簡介 

TBE 緩沖液是生物學(xué)中常使用的核酸電泳緩沖鹽溶液，主要用于 DNA 的瓊脂糖凝膠電泳。TBE 的主要成分是 Tris-硼酸鹽與 EDTA，緩沖能力強(qiáng)，適合較長時(shí)間電泳，分辨率較高，電泳小于 1kb 的片段時(shí)分離效果好。

TBE 緩沖液中的硼酸成分會(huì)影響 DNA 回收效率以及后續(xù)的酶反應(yīng)，若要進(jìn)行 DNA 片段的瓊脂糖凝膠電泳回收實(shí)驗(yàn)，建議使用 TAE 緩沖液。

本產(chǎn)品為 5×濃縮液，工作濃度為 0.5×，可直接用蒸餾水稀釋 10 倍后使用。若產(chǎn)品出現(xiàn)沉淀析出，請置于37℃水浴使其溶解，不影響使用。

About TBE Buffer

Tris buffers are used under slightly basic pH conditions, as for DNA electrophoresis, because this keeps the DNA soluble in the solution and deprotonated so it will be attracted to the positive electrode and will migrate through a gel. EDTA is an ingredient in the solution because this common chelating agent protects nucleic acids from degradation by enzymes. The EDTA chelates divalent cations that are cofactors for nucleases that may contaminate the sample. However, since the magnesium cation is a cofactor for DNA polymerase and restriction enzymes, the concentration of EDTA is kept purposely low (aroun 1 mM concentration).

Although TBE and TAE are common electrophoresis buffers, there are other options for low-molarity conductive solutions, including lithium borate buffer and sodium borate buffer. The problem with TBE and TAE is that Tris-based buffers limit the electric field that can be used in electrophoresis because too much charge causes a runaway temperature.