貨號 PH1755

名稱 Tris-硼酸電泳緩沖液（10×TBE）TBE Buffer,10×

規格 500ml

存儲 Store at RT ,12個月有效。
 
產品簡介 

TBE 緩沖液是生物學中常使用的核酸電泳緩沖鹽溶液，主要用于DNA的瓊脂糖凝膠電泳。TBE的主要成分是Tris-硼酸鹽與EDTA，緩沖能力強，適合較長時間電泳，分辨率較高，電泳小于1kb的片段時分離效果好。TBE 緩沖液中的硼酸成分會影響DNA回收效率以及后續的酶反應，若要進行DNA片段的瓊脂糖凝膠電泳回收實驗，建議使用TAE 緩沖液。

About TBE Buffer

Tris buffers are used under slightly basic pH conditions, as for DNA electrophoresis, because this keeps the DNA soluble in the solution and deprotonated so it will be attracted to the positive electrode and will migrate through a gel. EDTA is an ingredient in the solution because this common chelating agent protects nucleic acids from degradation by enzymes. The EDTA chelates divalent cations that are cofactors for nucleases that may contaminate the sample. However, since the magnesium cation is a cofactor for DNA polymerase and restriction enzymes, the concentration of EDTA is kept purposely low (aroun 1 mM concentration).

Although TBE and TAE are common electrophoresis buffers, there are other options for low-molarity conductive solutions, including lithium borate buffer and sodium borate buffer. The problem with TBE and TAE is that Tris-based buffers limit the electric field that can be used in electrophoresis because too much charge causes a runaway temperature.